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5 avril 2011 2 05 /04 /avril /2011 18:11

Nature Reviews Gastroenterology and Hepatology 8, 182 (April 2011) doi:10.1038/nrgastro.2011.26

            IBD: Modified Lactobacillus acidophilus may regulate intestinal inflammation

Abstract

Lactobacillus acidophilus that lack lipoteichoic acid (a major component of the cell walls of Gram-positive bacteria) have an anti-inflammatory effect in mice that results in resistance to colitis, according to a US research group.“The gastrointestinal microbiota and the immune system are closely tied, and constantly influence each other,” explains lead author Mansour Mohamadzadeh from Northwestern University, Chicago, IL.

 

Proc Natl Acad Sci U S A. 2011 Mar 15;108 Suppl 1:4623-30. Epub 2011 Jan 31.

Regulation of induced colonic inflammation by Lactobacillus acidophilus deficient in lipoteichoic acid.

Mohamadzadeh M, Pfeiler EA, Brown JB, Zadeh M, Gramarossa M, Managlia E, Bere P, Sarraj B, Khan MW, Pakanati KC, Ansari MJ, O'Flaherty S, Barrett T, Klaenhammer TR.

Northwestern University, The Feinberg School of Medicine, Chicago, IL 60611, USA. m.zadeh@northwestern.edu

Abstract

Imbalance in the regulatory immune mechanisms that control intestinal cellular and bacterial homeostasis may lead to induction of the detrimental inflammatory signals characterized in humans as inflammatory bowel disease. Induction of proinflammatory cytokines (i.e., IL-12) induced by dendritic cells (DCs) expressing pattern recognition receptors may skew naive T cells to T helper 1 polarization, which is strongly implicated in mucosal autoimmunity. Recent studies show the ability of probiotic microbes to treat and prevent numerous intestinal disorders, including Clostridium difficile-induced colitis. To study the molecular mechanisms involved in the induction and repression of intestinal inflammation, the phosphoglycerol transferase gene that plays a key role in lipoteichoic acid (LTA) biosynthesis in Lactobacillus acidophilus NCFM (NCK56) was deleted. The data show that the L. acidophilus LTA-negative in LTA (NCK2025) not only down-regulated IL-12 and TNFα but also significantly enhanced IL-10 in DCs and controlled the regulation of costimulatory DC functions, resulting in their inability to induce CD4(+) T-cell activation. Moreover, treatment of mice with NCK2025 compared with NCK56 significantly mitigated dextran sulfate sodium and CD4(+)CD45RB(high)T cell-induced colitis and effectively ameliorated dextran sulfate sodium-established colitis through a mechanism that involves IL-10 and CD4(+)FoxP3(+) T regulatory cells to dampen exaggerated mucosal inflammation. Directed alteration of cell surface components of L. acidophilus NCFM establishes a potential strategy for the treatment of inflammatory intestinal disorders.

 

PMID: 21282652 [PubMed - in process]PMCID: PMC3063598 [Available on 2011/9/15]

 

 

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5 avril 2011 2 05 /04 /avril /2011 09:59

« Trisomie 21 : premier essai thérapeutique

encourageant » , un puissant antioxydant extrait du thé vert

 

« la trisomie 21 pourrait bientôt être partiellement corrigée », selon des spécialistes de la maladie réunis aux deuxièmes Journées internationales Jérôme Lejeune, qui viennent de se dérouler à l’Institut Pasteur de Paris.


« plusieurs avancées récentes ont ouvert de nouveaux espoirs de modifier le cours de la maladie », expliquant qu’« en 2009, l’équipe de Jean-Maurice Delabar de l’université Paris-Diderot a montré que l’inhibition dès la naissance d’une enzyme excédentaire présente sur le chromosome 21 (la Dyrk1a) corrigeait tous les symptômes cognitifs de la maladie chez la souris ».


 

« Les chercheurs ont découvert qu’ils pouvaient utiliser pour cela un inhibiteur naturel de l’enzyme, un puissant antioxydant extrait du thé vert, le gallate d’épigallocatéchine ou EGCG. Cette substance naturelle a l’avantage supplémentaire de ne présenter aucun effet secondaire et de pouvoir être administré par voie orale ».

 « en 2010, l’équipe du Pr Mara Dierssen, du Centre sur la régulation génomique à Barcelone en Espagne, a lancé un premier essai clinique entièrement financé par la Fondation Jérôme Lejeune. Ce premier test chez l’homme paraît déjà très positif ».


 

Mara Dierssen déclare ainsi que « 3 mois après le début du traitement, la plupart des familles avaient deviné si leur enfant trisomique recevait l’EGCG ou un placebo, et 1 mois de traitement semble suffire pour observer un effet positif sur la mémoire et la psychomotricité. Cet effet disparaît avec l’arrêt du traitement ».

 

La spécialiste précise n’avoir observé « aucun effet indésirable ».

 

Le quotidien souligne que « la puissante action de cette molécule s’explique par le rôle clé joué par l’enzyme Dyrk1a dans de nombreux processus cellulaires et cela à tous les niveaux de l’organisme », puis note qu’« après cet essai pilote effectué auprès de 30 patients âgés de 18 à 26 ans, les chercheurs espagnols vont lancer une grande étude internationale dans une dizaine de centres hospitaliers ».

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5 avril 2011 2 05 /04 /avril /2011 09:53

« Des chercheurs percent le secret du choc anaphylactique »


C’est ce qu’annonce La Croix, qui rend compte sur quelques lignes des travaux de Pierre Bruhns et de son équipe de l’Inserm-Institut Pasteur Paris, avec des chercheurs de l’université de Tokyo (Japon).


Le journal note qu’« on pensait que l’anaphylaxie était due à la mobilisation de certains anticorps (immunoglobines E) et certains globules blancs (mastocytes et basophiles) capables de libérer en quelques minutes de puissants médiateurs de l’inflammation des tissus, l’histamine et le PAF ».


« L’équipe menée par Pierre Bruhns vient de découvrir qu’elle est due à d’autres anticorps et d’autres globules blancs. Ils envisagent un essai clinique pour 2014 avec l’hôpital Bichat. S’ils ont assez d’argent », observe La Croix.


Le chercheur remarque en effet qu’en matière de recherche clinique, « l’immunologie est un peu le parent pauvre par rapport au cancer ou aux maladies infectieuses ».

J Clin Invest. 2011 Mar 23. pii: 45232. doi: 10.1172/JCI45232. [Epub ahead of print]

Mouse and human neutrophils induce anaphylaxis.

Jönsson F, Mancardi DA, Kita Y, Karasuyama H, Iannascoli B, Van Rooijen N, Shimizu T, Daëron M, Bruhns P.

 

Abstract

 

Anaphylaxis is a life-threatening hyperacute immediate hypersensitivity reaction. Classically, it depends on IgE, FcεRI, mast cells, and histamine.

However, anaphylaxis can also be induced by IgG antibodies, and an IgG1-induced passive type of systemic anaphylaxis has been reported to depend on basophils.

In addition, it was found that neither mast cells nor basophils were required in mouse models of active systemic anaphylaxis.

Therefore, we investigated what antibodies, receptors, and cells are involved in active systemic anaphylaxis in mice.

We found that IgG antibodies, FcγRIIIA and FcγRIV, platelet-activating factor, neutrophils, and, to a lesser extent, basophils were involved.

Neutrophil activation could be monitored in vivo during anaphylaxis.

Neutrophil depletion inhibited active, and also passive, systemic anaphylaxis.

Importantly, mouse and human neutrophils each restored anaphylaxis in anaphylaxis-resistant mice, demonstrating that neutrophils are sufficient to induce anaphylaxis in mice and suggesting that neutrophils can contribute to anaphylaxis in humans.

Our results therefore reveal an unexpected role for IgG, IgG receptors, and neutrophils in anaphylaxis in mice.

 

These molecules and cells could be potential new targets for the development of anaphylaxis therapeutics if the same mechanism is responsible for anaphylaxis in humans.

 

PMID: 21436586 [PubMed - as supplied by publisher]

 

 

 

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5 avril 2011 2 05 /04 /avril /2011 08:08

 

 

http://arxiv.org/pdf/1012.5166

 

Abstract.

 

Some bacterial and viral DNA sequences have been found to induce low frequency electromagnetic waves in high aqueous dilutions.

 

This phenomenon appears to be triggered by the ambient electromagnetic background of very low frequency.

 

We discuss this phenomenon in the framework of quantum field theory. A scheme able to account for the observations is proposed.

 

The reported phenomenon could allow to develop highly sensitive detection systems for chronic bacterial and viral infections.

 

 

Conclusions and medical applications

 

 

In this paper we have described the experiments showing a new property of DNA and the induction of electromagnetic waves in water dilutions.

 

We have briefly depicted the theoretical scheme which can explain qualitatively the features observed in these experiments.


We remark that it is possible to detect the same EMS from the plasma of patients suffering from various infections and chronic diseases.

 

The plasma has to be kept fresh and unfrozen.

 

If the plasma is frozen at −70 C then one must extract the DNA in order to recover the signals. The DNA can be also extracted from tissue biopsies.

 

The list of diseases in which EMS have been found (such as Alzheimer, Parkinson, Multiple Sclerosis, various neuropathies, chronic Lyme syndrome, rheumatoid arthritis) indicate clearly that their presence is not limited to diseases known to be of infectious origin:


 

The fact that EMS have been found in diseases not known to be of infectious origin is intriguing, and leads us to seek bacterial or viral factors in these diseases.

 

A special case is that of HIV. Signals have been regularly detected coming from HIV DNA sequences in the blood of patients treated by antiretroviral therapy and responding well to that treatment by the disappearance of viral RNA copies in the circulating blood.

 

This would indicate that such DNA comes from a reservoir not accessible to the classical treatment, and not from viral particles circulating in the blood.

 

Moreover, not only the plasma of the patients, but also the red blood cell fraction contains DNA emitting signals.

 

This is intriguing, as the red cells do not contain any cellular DNA, and as the virus does not bind to erythrocyte membrane.

 

The possibility that a third element is involved is under investigation.

 

The hypothesis [2] has been put forward that the antiretroviral treatment, which includes reverse transcriptase inhibitors, is itself selecting for a new way of viral DNA replication involving one or several cellular DNA polymerases.

 

As for the M. pirum DNA, it is suggested that the HIV DNA fragments and their nanostructures present in the blood may not originate from cell lysis but, on the contrary, represent pieces of definite size able to recombine in the appropriate recipient cells (lymphocytes) to form whole genomic DNA and finally regenerate infectious virus.

 

Whatever is the origin of this DNA, its easy detection by electromagnetic signals may render it a unique biomarker for attacking the viral reservoir. Its existence in the blood by more classical PCR technology has also been confirmed [2].

 

One thus has a powerful tool to test new types of treatment aimed at eradicating HIV infection, which has never yet been achieved, by the treatments actually in use.

 

This is particularly important for patients in countries in which HIV prevalence is very high, (5 − 10 % in a large part of sub-Saharan Africa).

 

The process of launching clinical trials inWest and South Africa to test new therapeutics is planned.

 

Their efficacy will be monitored by this new test, together with the improvement of more classical parameters, evaluating the full restoration of the immune system.

 

The objective here is eradication of HIV infection, so that it will not be necessary for patients to be treated for life by a combination of toxic and expensive drugs.

 

Our work is interdisciplinary, involving biologists, physicists, and medical doctors. There are of course many unresolved questions raised by our findings, which deserve more work and more interactions.

 

DNA signalling is stimulated by 7 Hz naturally occurring waves on earth. Waves produced by the human brain are also in the range of 7 Hz. These are interesting questions to be asked and possibly answered.

 


References


[1] Montagnier L, A ̈ıssa J, Ferris S, Montagnier J-L and Lavallee C 2009 Interdiscip. Sci. Comput. Life Sci. 1 81–90

[2] Montagnier L, A ̈ıssa J, Lavallee C, Mbamy M, Varon J and Chenal H 2009 Interdiscip. Sci. Comput. Life Sci. 1 245–253

[3] Montagnier L 2010 Lindau Nobel Conference [4] Nickolaenko A P and Hayakawa M 2002 Resonances in the Earth-ionosphere cavity (Dordrecht-Boston-

London: Kluwer Academic Publishers) [5] Gibbon D G et al 2010 Science 329 52–56 [6] Preparata G 1995 QED Coherence in matter (Singapore: World Scientific) [7] Arani R, Bono I, Del Giudice E, Preparata G 1995 Int. J. Mod. Phys. B 9 1813–1841 [8] Del Giudice E, Preparata G and Vitiello G 1988 Phys. Rev. Lett. 61 1085–1088 [9] Del Giudice E and Vitiello G 2006 Phys. Rev. A 74 022105

[10] Del Giudice E and Tedeschi A 2009 Electr. Biol. Med. 26 48–54 [11] Del Giudice E, Spinetti P R and Tedeschi A 2010 Water 2 566–586 (online Journal) [12] Del Giudice E, Pulselli R.M and Tiezzi E 2009 Ecol. Model 220 1874–1879 [13] Marchettini N, Del Giudice E, Voeikov and V L and Tiezzi E 2010 J. Theo. Bio. 265 511–516 [14] Voeikov V.L, Del Giudice E 2009 Water Journal.org. 1 52–75 [15] Landau L.D 1941 J. Physics USSR (Moscow) 5 71 [16] Szent-Gyorgyi A 1957 Bioenergetics (New York, NY: Academic Press Inc) [17] Zhadin M. N, Novikov V V, Barnes F S and Pergola N F 1998 Bioelectromagn. 19(1) 41–49 [18] Zhadin M and Giuliani L 2006 Electr. Biol. Med. 25(4) 269–280 [19] Del Giudice E, Fleischmann M, Preparata G and Talpo G 2002 Bioelectromagnetics 27 522–530. [20] Del Giudice E and Giuliani L 2010 Eur. J. of Oncol. Library 5

7–23

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5 avril 2011 2 05 /04 /avril /2011 08:07

 

 

 

http://www.21stcenturysciencetech.com/Articles_2011/Winter-2010/Montagnier.pdf

 

 

 

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5 avril 2011 2 05 /04 /avril /2011 08:04
What is Life? A Non-Particle View
Interview sur  
http://www.larouchepac.com/node/17802
March 21st, 2011 • 8:41 PM

Can life be reduced to nothing more than an interesting arrangement of atoms and molecules? No. LPAC Basement research team member Oyang Teng and 21st Century Science & Technology editor Laurence Hecht discuss the implications of Luc Montagnier's recent experiments demonstrating water-mediated, low-frequency electromagnetic emission from bacterial and viral DNA. Preceding the discussion is a 10-minute animated segment on Montagnier's experiments.

See also: Laurence Hecht, "New Evidence for a Non-Particle View of Life."

 


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3 avril 2011 7 03 /04 /avril /2011 21:33

 

JOURNEE MONDIALE DE L’AUTISME : MISE EN LIGNE DU SITE ET DE LA PREMIERE

VIDEO DE L’ALLIANCE MONDIALE POUR L’AUTISME 

 

Communiqué de presse                   

SAN DIEGO, CALIFORNIE—En ce 2 avril 2011, Journée mondiale de l’autisme, l’Alliance mondiale pour l’autisme (GAA, Global Autism Alliance) lance son site internet, www.GlobalAutismAlliance.com, et présente une vidéo coproduite à travers le monde. L’Alliance mondiale pour l’autisme est le fruit d’une collaboration de représentants et associations pour l’autisme en Australie, au Canada, au Chili, en France, en Inde, en Italie, au Japon, au Mexique, au Nigéria, au Puerto Rico et aux Etats-Unis. 

L’Alliance mondiale pour l’autisme a pour objectif de diffuser une information de qualité sur les diagnostics, les traitements et l’éducation auprès des gouvernements et des organismes, des parents ainsi que du grand public. Pour ce faire, l’Alliance organisera des wébinaires et des congrès internationaux à l’adresse des professionnels et des parents, traduira des documents importants, et suscitera la mise en réseau de centres médicaux et autres organismes à travers le monde.

Selon une étude américaine, les troubles du spectre autistique concernent un enfant sur 110 ; une prévalence probablement constante à travers le monde.

L’autisme est une pathologie potentiellement traitable, or la majorité des sujets qui en sont atteints ne bénéficie pas de traitements adéquats. Nombre de personnes avec trouble autistique présentent des affections telles que problèmes gastro-intestinaux, dysfonctionnements immunitaires et anomalies métaboliques. Des approches telles que la nutrithérapie, certains régimes d’éviction, des programmes éducatifs spécifiques, entre autres, constituent pour ces personnes une aide efficace. 

Il est à déplorer que les gouvernements et les professionnels à travers le monde prennent aussi peu acte des recherches menées, qui documentent les problèmes médicaux associés aux troubles du spectre autistique et l’apport de telles approches. Rares sont les médecins, les thérapeutes et les éducateurs suffisamment sensibilisés à ces caractéristiques particulières des troubles autistiques.

L’Alliance mondiale pour l’autisme souhaite améliorer la qualité de vie des personnes vivant avec un trouble autistique et de leurs familles en attirant l’attention, à travers le monde, sur la nécessité de diagnostics et sur l’existence de traitements et d’accompagnements éducatifs efficaces.

 

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3 avril 2011 7 03 /04 /avril /2011 09:25

As standards of living improve, so does attention to hygiene. However, it seems there may be a danger in being too diligent about it.


To test the hygiene hypothesis, researchers have found a unique 'living laboratory' at the border between Russian Karelia and Finland and nearby Estonia, which is quickly raising living standards. The three populations present a unique opportunity to test gene-environmental interactions in the development of immune-mediated diseases. At the onset of the study, the incidence of type 1 diabetes (T1D) was noted as being six times lower in Russian Karelia than in Finland.

The 'Pathogenesis of Type 1 Diabetes - Testing the Hygiene Hypothesis' (Diabimmune) project aims to assess the role of hygiene in the development of immune-mediated diseases, T1D in particular. Also, it is working to determine how the presence of infectious agents can actually offer a protective effect.

The project's aims from the start were to compare the frequencies of autoimmunity, signs of allergy and various infections, germs in the intestines and the dietary intake of young children in all three populations. This cohort has gathered 1 668 young children aged between 3 and 5, while the birth cohort totals some 8 409 infants from birth to 3 years of age.

The birth cohort aims at detailing the development of the immune system from birth to adulthood with tools that describe gene functions and interactions (functional genomics). Also, in this cohort, researchers will compare the functional characteristics of regulatory T cells. These are white blood cells that work to stop the immune system from being wrongly activated.

The Diabimmune Core Laboratory in Helsinki, Finland has recently completed an analysis of 1 787 biological samples for diabetes-associated autoantibodies. The National Institute for Welfare and Health is examining cord and peripheral blood samples and a centre in Petrozavodsk, Russia is conducting flow cytometry studies.

At the University of Turku in Finland, project members are concentrating on isolation and quality control of ribonucleic acid.

Also in Finland, the University of Tampere, responsible for dietary studies, has succeeded in developing a test platform for screening germs from stool, serum and nasal swab samples.

It has already carried out analyses of the samples collected.

Academic Hospital Groningen in the Netherlands is studying colonies of intestinal germs in infants living across the spectrum of standards of hygiene.


Country: FINLAND

Information Source: Result from the EU funded FP7-HEALTH programme

Date: 2011-03-25

Contact Details
VAINIO-MATTILA, Katariina (Ms)
HELSINGIN YLIOPISTO
Research Affairs Unit
Research Liaison Officer
8, Haartmaninkatu PO Box 63
00014
HELSINKI
FINLAND
Tel: +35-89191-25043
Fax: +35-89191-25044
Email: Contact
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Lyme Disease Update

 
Can antibiotics catch Lyme disease before it develops?

Do antibiotics alleviate the lingering symptoms after the infection is cured?

Two recent studies published in The New England Journal of Medicine shed light on these two controversial questions.

In the first study, conducted in Westchester County, New York, an area with high rates of Lyme disease, researchers gave a single dose of either an antibiotic or a placebo to patients who were bitten by deer ticks in the previous 72 hours. 

The researchers found the antibiotic doxycycline prevented the disease form developing. However, only 3.2% of the untreated patients developed the characteristic bull's eye rash around the tick bite.

This suggests the risk of Lyme disease is low even in Westchester County. The study also found the infection was more likely to develop from bites by ticks in the nymph stage, and if the tick was attached for 72 hours or more.

This study suggests preventive treatment is only worthwhile in areas with a particularly high incidence of the disease or if you are bitten by a nymphal deer tick that has been attached for three days or more. You should save the tick in a jar of alcohol so it may be identified to help you and your physician make that decision.

In the second study, researchers investigated whether prolonged treatment with antibiotics reduces the fatigue, muscle and joint pain, and mood or memory disturbances that some people experience even after the infection has cleared.

But the study was discontinued early when preliminary results showed prolonged antibiotic treatment was no more effective than a placebo at improving the persistent symptoms. 

If you are experiencing post-Lyme disease symptoms, long-term use of antibiotics is unlikely to help. But there is hope your condition will improve — the study found symptoms improved in 36% of the untreated patients. 

 


September 2001 Update   Harvard Medical School

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1 avril 2011 5 01 /04 /avril /2011 16:12

State

2007

2008

 

Confirmed

Probable*

Alabama

13

6

3

Alaska

10

6

0

Arizona

2

2

6

Arkansas

1

0

0

California

75

74

0

Colorado

0

2

1

Connecticut

3058

2738

1158

Delaware

715

772

0

DC

116

71

3

Florida

30

72

16

Georgia

11

35

0

Hawaii

0

0

0

Idaho

9

5

4

Illinois

149

108

0

Indiana

55

42

0

Iowa

123

85

24

Kansas

8

16

0

Kentucky

6

5

0

Louisiana

2

3

0

Maine

529

780

128

Maryland

2576

1746

472

Massachusetts

2988

3960

622

Michigan

51

76

16

Minnesota

1238

1046

236

Mississippi

1

1

0

Missouri

10

6

0

Montana

4

6

11

Nebraska

7

8

4

Nevada

15

9

3

New Hampshire

896

1211

390

New Jersey

3134

3214

271

New Mexico

5

4

4

New York

4165

5741

2053

North Carolina

53

16

31

North Dakota

12

8

2

Ohio

33

40

5

Oklahoma

1

1

1

Oregon

6

18

20

Pennsylvania

3994

3818

0

Rhode Island

177

186

24

South Carolina

31

14

15

South Dakota

0

3

0

Tennessee

31

7

24

Texas

87

105

48

Utah

7

3

2

Vermont

138

330

74

Virginia

959

886

47

Washington

12

22

1

West Virginia

84

120

15

Wisconsin

1814

1493

541

Wyoming

3

1

2

U.S. Total

27,444

28,921

6,277

*2008 revision of the National Surveillance Case Definition allows for reporting of probable cases

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