Assessment system for dioxin absorption in the small intestine and prevention of its absorption by food factors.
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
It has been reported that 90% of the amount of dioxin in the whole body is absorbed orally with food.
However, a concise and simple system to assess dioxin absorption in the small intestine has not yet been established.
The present study reports a new in vitro assessment system for this purpose. A stable dioxin-responsive cell line was established by introducing a plasmid that incorporates a xenobiotic-responsive element upstream of the luciferase gene into human hepatic HepG2 genomic DNA.
Dioxin was added to the apical side of differentiated human intestinal epithelial Caco-2 cell monolayers that had been cultured on a semipermeable membrane, and the basal medium was recovered after an appropriate incubation time.
To the recovered medium was added dioxin-responsive HepG2, and a luciferase assay was performed.
The established stable cell line clearly showed dose-and time-dependent response to dioxin.
When a food factor such as chlorophyll, which has been reported to increase dioxin excretion in in vivo studies, was added with dioxin, a significant decrease in dioxin permeability to the Caco-2 monolayer was observed.
This assessment system would be useful to search for those food factors that could prevent dioxin absorption in the small intestine.
PMID: 15630229 [PubMed - indexed for MEDLINE]