Chers amis consoeurs et confrères
Dear friends and colleagues
Voici le poster et l’abstract de la présentation que l’équipe de Phelix / Université de Leicester va faire à l’ILADS le vendredi 5 octobre.
Nous avons bien progressé dans la mise au point d’un test PCR (double amorce ADN de phage) plus sensible et plus spécifique..
Les premiers résultats du pouvoir lytique de lysines et holines de phages spécifiques seront également montrés . L’on vous tiendra informés des autres étapes
Please find herewith the poster and the abstract that Phelix /leicester university team is going to present at ILADS meeting ( october 5th)
We have made a to of progress in the research and development of a specific and sensitive PCR phage based test and first in vitro results on phage lysins and holines are also promising
Hope we will see you in Philadelphia ; We will keep you posted
Dr Louis Teulières
230 Rue du Faubourg Saint Honoré 75008
Tel +33 (0)174900407
Phelix - London & Paris
(chronic infections and bacteriophages research group)
@Phelix_RD / Phelix Charity
Is there a place for bacteriophages in diagnosis and treatment of Lyme Disease?
Jinyu Shan1, Louis Teulières2,
and Martha Clokie1 1Department of Infection, Immunity, and Inflammation, University of Leicester, LE1 6RH, UK 2Neurological disabilities Center, Fondation A De Gaulle, Versailles, 78000, France
Bacteriophages (phages) are viruses that attack and kill bacteria. They are used effectively to target many bacterial infections but have been little studied in Borrelia. The current primary treatment for Lyme Disease (LD) is antibiotic therapy but complete Borrelia eradication is difficult. In addition, the current diagnosis of LD is problematic. Phages offer a promising new approach for both LD diagnosis and treatment. In this project, we aim to develop a phage-based diagnostic method to detect Borrelia and to identify and develop phages for treatment purposes.
We are pursuing four approaches; (1) Borrelia phage genomes have been analysed and used to design a phage-based diagnostic, which has been tested on ~200 patient serum samples which are positive, borderline or negative, with western blot analysis. (2) tick gut samples have been used for phage isolation on five dominant Lyme Borrelia strains. (3) phages residing within Borrelia genomes have been induced from their bacterial hosts using Mitomycin C. (4) phage-encoded holins (enzymes that rupture bacterial cytoplasmic membranes) and endolysins (enzymes that break down bacterial cell walls) have been investigated to establish ‘anti-Borrelia’ characteristics.
A Borrelia phage-based diagnostic method was developed and validated against human serum samples. This method showed 100% specificity and ≥75% sensitivity. The test takes ~2 hours, can detect Lyme from 400 µl serum, and distinguish Lyme from Relapsing Fever. Borrelia has been identified from ten tick samples of the 138 tested and phage identification is in progress. Phages have been induced from 7 Borrelia strains. Holins and endolysins have been over-expressed in a yeast-based protein expression system. We are currently working on their purification.
The phage-based diagnostic method is quick, highly sensitive, and distinguishes Lyme from Relapsing Fever. Borrelia phages can be induced from strains and novel methods have been optimised to isolate them from ticks.
Poster : file:///C:/Users/JPL/Desktop/Jinyu%20Shan-Philadelphia%20Nove%202016%20poster.pdf