Lyme Borreliosis - the Utility of Improved Real-Time PCR Assay in the Detection of Borrelia burgdorferi Infections.
Bil-Lula I, et al. Adv Clin Exp Med. 2015 Jul-Aug.
Show full citation
BACKGROUND: Infections of Borrelia burgdorferi sensu lato reveal clinical manifestations affecting numerous organs and tissues. The standard diagnostic procedure of these infections is quite simple if a positive history of tick exposure or typical erythema migrans appears. Lack of unequivocal clinical symptoms creates the necessity for further evaluation with laboratory tests.
OBJECTIVES: This study discusses the utility of a novel, improved, well-optimized, sensitive and highly specific quantitative real-time PCR assay for the diagnostics of infections caused by Borrelia burgdorferi sensu lato.
MATERIAL AND METHODS: We designed an improved, specific, highly sensitive real-time quantitative polymerase chain reaction (RQ-PCR) assay for the detection and quantification of all Borrelia burgdorferi genotypes. A wide validation effort was undertaken to ensure confidence in the highly sensitive and specific detection of B. burgdorferi.
RESULTS: Due to high sensitivity and great specificity, as low as 1.6 × 10² copies of Borrelia per mL of whole blood could be detected. As much as 12 (3%) negative ELISA IgM results, 14 (2.8%) negative results of Line blot IgM, 11 (3.1%) and 7 (2.7%) of negative ELISA IgG and Line blot IgG results, respectively, were positive in real-time PCR.
CONCLUSIONS: The data in this study confirms the high positive predictive value of real-time PCR test in the detection of Borrelia infections.
PMID 26469112 [PubMed)
commenter cet article